SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Viscardi,Lucas Henriques; Tovo-Rodrigues,Luciana; Paré,Pamela; Fagundes,Nelson Jurandi Rosa; Salzano,Francisco Mauro; Paixão-Côrtes,Vanessa Rodrigues; Bau,Claiton Henrique Dotto; Bortolini,Maria Cátira.

Abstract The FOXP subfamily is probably the most extensively characterized subfamily of the forkhead superfamily, playing important roles in development and homeostasis in vertebrates. Intrinsically disorder protein regions (IDRs) are protein segments that exhibit multiple physical interactions and play critical roles in various biological processes, including regulation and signaling. IDRs in proteins may play an important role in the evolvability of genetic systems. In this study, we analyzed 77 orthologous FOXP genes/proteins from Tetrapoda, regarding protein disorder content and evolutionary rate. We also predicted the number and type of short linear motifs (SLIMs) in the IDRs. Similar levels of protein disorder (approximately 70%) were found for...

Homo sapiens, Homo neanderthalensis and the Denisova specimen: new insights on their evolutionary histories using whole-genome comparisons

Palavras-chave: FOXP2; Intrinsically disordered protein regions; Forkhead superfamily; Short linear motif; Molecular evolution.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100181

Paixão-Côrtes,Vanessa Rodrigues; Viscardi,Lucas Henrique; Salzano,Francisco Mauro; Hünemeier,Tábita; Bortolini,Maria Cátira.

After a brief review of the most recent findings in the study of human evolution, an extensive comparison of the complete genomes of our nearest relative, the chimpanzee (Pan troglodytes), of extant Homo sapiens, archaic Homo neanderthalensis and the Denisova specimen were made. The focus was on non-synonymous mutations, which consequently had an impact on protein levels and these changes were classified according to degree of effect. A total of 10,447 non-synonymous substitutions were found in which the derived allele is fixed or nearly fixed in humans as compared to chimpanzee. Their most frequent location was on chromosome 21. Their presence was then searched in the two archaic genomes. Mutations in 381 genes would imply radical amino acid changes, with...

Interethnic admixture and the evolution of Latin American populations

Palavras-chave: Human evolution; Comparative genomics; Positive selection; Neanderthal; Denisova.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600003

Salzano,Francisco Mauro; Sans,Mónica.

A general introduction to the origins and history of Latin American populations is followed by a systematic review of the data from molecular autosomal assessments of the ethnic/continental (European, African, Amerindian) ancestries for 24 Latin American countries or territories. The data surveyed are of varying quality but provide a general picture of the present constitution of these populations. A brief discussion about the applications of these results (admixture mapping) is also provided. Latin American populations can be viewed as natural experiments for the investigation of unique anthropological and epidemiological issues.

LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens

Palavras-chave: Interethnic admixture; Latin America; Population structure.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200003

Lima-Rosa,Carlos André da Veiga; Canal,Cláudio Wageck; Fallavena,Paulo Roberto Vargas; Freitas,Loreta Brandão de; Salzano,Francisco Mauro.

A total of 149 chickens from two different sources (one non-commercial, the other commercial) was tested for variability of the LEI0258 microsatellite. Fifty- three genotypes, explainable by 15 alleles, were found. There are clear allele and heterozygosity differences between the two samples. One of them was simultaneously studied for the MHC B-F haplotypes. Strong genetic disequilibrium was observed between the variants of the two systems, therefore providing a cheap alternative for MHC genotyping.

New evidence for balancing selection at the HLA-G locus in South Amerindians

Palavras-chave: Microsatellite; LEI0258; B-F haplotypes; Brazilian chickens.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300008

Veit,Tiago Degani; Cazarolli,Juciana; Salzano,Francisco Mauro; Schiengold,Marion; Chies,José Artur Bogo.

HLA-G is a non-classical HLA (Human Leukocyte Antigen) molecule characterized by limited tissue distribution under normal physiological conditions and low variability at both DNA and protein levels. Several studies suggest that HLA-G could play a role, as an immunoregulatory molecule, in situations as diverse as transplantation, cancer, viral infections and inflammatory diseases. A total of 237 individuals from 21 South American tribes speaking nine different linguistic families were studied in relation to the 14 bp insertion/deletion polymorphism at the HLA-G gene. A consistent (seven in nine) excess of heterozygosity in samples classified by language was obtained. Our data supply evidences for balancing selection acting at the HLA-G 14 bp INDEL region....

Oxytocin and arginine vasopressin receptor evolution: implications for adaptive novelties in placental mammals

Palavras-chave: HLA-G 14 bp INDEL; Balancing selection; Amerindians; Pathogen resistance.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600005

Paré,Pamela; Paixão-Côrtes,Vanessa R.; Tovo-Rodrigues,Luciana; Vargas-Pinilla,Pedro; Viscardi,Lucas Henriques; Salzano,Francisco Mauro; Henkes,Luiz E.; Bortolini,Maria Catira.

Abstract Oxytocin receptor (OXTR) and arginine vasopressin receptors (AVPR1a, AVPR1b, and AVPR2) are paralogous genes that emerged through duplication events; along the evolutionary timeline, owing to speciation, numerous orthologues emerged as well. In order to elucidate the evolutionary forces that shaped these four genes in placental mammals and to reveal specific aspects of their protein structures, 35 species were selected. Specifically, we investigated their molecular evolutionary history and intrinsic protein disorder content, and identified the presence of short linear interaction motifs. OXTR seems to be under evolutionary constraint in placental mammals, whereas AVPR1a, AVPR1b, and AVPR2 exhibit higher evolutionary rates, suggesting that they...

Freitas,Loreta Brandão de; Koehler-Santos,Patrícia; Salzano,Francisco Mauro.

Palavras-chave: Oxytocin receptor; Arginine vasopressin receptors; Molecular evolution; Protein disorder; Interaction motifs.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400646

Ciência Rural

Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous...

Searching for ancient balanced polymorphisms shared between Neanderthals and Modern Humans

Palavras-chave: Triticum aestivum L.; Defense proteins; Genetic variability.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016

Viscardi,Lucas Henriques; Paixão-Côrtes,Vanessa Rodrigues; Comas,David; Salzano,Francisco Mauro; Rovaris,Diego; Bau,Claiton Dotto; Amorim,Carlos Eduardo G.; Bortolini,Maria Cátira.

Abstract Hominin evolution is characterized by adaptive solutions often rooted in behavioral and cognitive changes. If balancing selection had an important and long-lasting impact on the evolution of these traits, it can be hypothesized that genes associated with them should carry an excess of shared polymorphisms (trans- SNPs) across recent Homo species. In this study, we investigate the role of balancing selection in human evolution using available exomes from modern (Homo sapiens) and archaic humans (H. neanderthalensis and Denisovan) for an excess of trans-SNP in two gene sets: one associated with the immune system (IMMS) and another one with behavioral system (BEHS). We identified a significant excess of trans-SNPs in IMMS (N=547), of which six of...

The first of a series of articles dedicated to the 60th anniversary of the Brazilian Society of Genetics (SBG)

Palavras-chave: Human behavior; Evolution; Balancing selection; Immune genes; Behavioral genes.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100067

Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M..

The second of a series of articles for the 60th anniversary of the Brazilian Society of Genetics

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300301

Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M..

The third of a series of articles for the 60th anniversary of the Brazilian Society of Genetics

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400475

Salzano,Francisco Mauro; Santos,Fabrício Rodrigues dos; Hartfelder,Klaus; Menck,Carlos F.M..

Uniparental genetic markers in South Amerindians

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100001

Bisso-Machado,Rafael; Bortolini,Maria Cátira; Salzano,Francisco Mauro.

A comprehensive review of uniparental systems in South Amerindians was undertaken. Variability in the Y-chromosome haplogroups were assessed in 68 populations and 1,814 individuals whereas that of Y-STR markers was assessed in 29 populations and 590 subjects. Variability in the mitochondrial DNA (mtDNA) haplogroup was examined in 108 populations and 6,697 persons, and sequencing studies used either the complete mtDNA genome or the highly variable segments 1 and 2. The diversity of the markers made it difficult to establish a general picture of Y-chromosome variability in the populations studied. However, haplogroup Q1a3a* was almost always the most prevalent whereas Q1a3* occurred equally in all regions, which suggested its prevalence among the early...